Signalling

Part:BBa_K5371006:Design

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-28)


GAL4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1349
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 706
    Illegal PstI site found at 1349
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI.rc site found at 1813
    Illegal SapI site found at 502


Design Notes

Incorporating GAL4/UAS system into production allows regulation of the upstream spontaneous oscillator to the producing plasmids.


Source

The GAL4 was amplified from genome of Saccharomyces cerevisiae.

References